早期应用凉血活血中药对大鼠放射性肺损伤中医证候学演变的影响

Effects of Pre-treatment of Chinese Medicine with Functions of Cooling and Promoting Blood Circulation on the TCM Syndrome of Rats with Radio-pulmonary Lesion

目的:评价和验证早期应用凉血活血法对放射性肺损伤的防治效果及其对证候学演变的影响。方法:将160只雌性Wister大鼠,随机分为单纯照射组(A)、中药治疗组(B)、中药对照组(C)、空白对照组(D)各40只,A组和B组给予X线照射3Gy×2次/周×(3～5周),C,D组不作X线照射。分别在第3、5、8、12、26周末,各组随机抽取8只处死。对大鼠活体、肺脏大体和肺组织切片进行动态观察、比较和分析。结果:A组在照射早期,大鼠表现为"热毒内盛"证,肺脏大体和镜下呈"热伤血络"证表现;后期肺脏呈"瘀血阻络"证表现。B组明显轻于A组,而C组及D组无异常变化。结论:早期应用凉血活血中药可以减轻放射性肺损伤,对其证候学演变起到抑制作用;动物实验可揭示放射性肺损伤的病理特征,为早期应用凉血活血法防治放射性肺损伤提供理论与实验依据。

Objective：To evaluate the effects of pre-treatment of Chinese Medicine with functions of cooling and promoting blood circulation on the pathological changes of rats with radio-pulmonary lesion, and validate its control efficiency on the TCM syndrome. Methods： 160 female wistar rats were divided randomly into the model group（A）, the Chinese medicine group（ B）, the Chinese medicine control group（C） and the blank control group（D） with 40 rats in each group. The rats in group A and B received X-ray radiation at 3Gy × 2/W × （3 - 5W）. 8 rats randomly selected from each group were sacrificed at the end of 3^th , 5^th , 8^th , 12^th , 26^th week respectively. The developmental characteristics of TCM were analyzed in radio-pulmonary lesion during exposure by dynamic examination and comparison. Results：In group A, in early stage of radiation exposure, signs and symptoms as ＂dominance of heat in the interior＂ syndrome were observed and signs as ＂collaterals damaged and symptoms as ＂emphraxis symptoms in group B showed by heat＂ syndrome were observed by microscope; in later stage of radiation exposure, signs of collaterals by blood stasis＂ syndrome were observed by microscope. All the signs and less serious than group A; and no abnormal changes were observed in group C and D.Conclusion： Pre-treatment with Chinese medicine of cooling and promoting blood can effectiv lesion and control the evolution of TCM syndrome. Symptomatological and developmental induced lung injury can be revealed in animal experiments at each levels. The study provides ely prevent radio-pulmonary characteristics theoretical and of radiation- experimental basis for pre-treatment with Chinese medicine of cooling and promoting blood to prevent and control radio-pulmonary lesion.