当归、黄芪注射液对鸡胚绒毛尿囊膜模型的毒性研究

Study on Toxicity of Angelica Sinensis Injection and Astragalus Membranaceus Injection in Chorioallantoic Membrane Model

目的:通过观察当归、黄芪注射液对鸡胚绒毛尿囊膜(CAM)模型的毒性,探讨当归、黄芪注射液利用CAM模型研究血管生成的可行性。方法:制备CAM模型,观察应用当归、黄芪注射液及不同配比后CAM模型的存活情况。结果:当归、黄芪注射液及不同配比对CAM模型的存活无毒性,与生理盐水对照组相比无显著性差异(P>0.05)。结论:可以利用CAM模型研究当归、黄芪注射液的促血管生成作用。

Objective：To explore the toxicity of Angelica sinensis injection （ASI） and Astragalus membranaceus injection （AMI） in the chick embryo chorioallantoic membrane （CAM） model. Methods： Fertilized eggs were incubated for 7 days at 37.8℃, and randomly divided into 6 groups, and then were respectively treated with NS （negative control）, ASI, AMI, and different ratio of ASI and AMI. After another 3 days of incubation, the number of living chick embryos were counted. Results：The survival of chick embryos could not be inhibited by ASI and AMI, and our assay showed that no difference between therapy groups and negative control （ P 〉 0.05）. Conclusion： ASI and AMI could be used to study angiogenesis by CAM.