益气通痹合剂抗胶原诱导的关节炎大鼠关节滑膜血管新生作用的研究

The Effect of Yiqi Tongbi Mixture on Angiogenesis of Synovial Membrane in CIA Rats

目的:探讨益气通痹合剂抗风湿的作用及其机制。方法:以Ⅱ型胶原加完全弗氏佐剂诱导的关节炎(CIA)大鼠为类风湿性关节炎模型,通过对关节滑膜病理改变、大鼠右后足关节影像学评估及对血管内皮生长因子(VEGF)、基质金属蛋白酶-1、2、3、9(MMPs)在不同处理大鼠血清中的表达,探讨该制剂的治疗效果。结果:益气通痹合剂组的滑膜组织新生血管明显减少,与模型组比较差异显著(P<0.05),关节影像学有改善;模型组大鼠血清VEGF、MMPs活性表达明显升高,益气通痹合剂组起下调作用。结论:益气通痹合剂能改善模型大鼠的病理学及影像学改变,并通过下调大鼠血清中VEGF、MMPs的表达而抑制CIA大鼠关节滑膜的血管新生功能。

Objective：To investigate the mechanism of Yiqi Tongbi Mixture on anti-rheumatoid in arthritic rats. Methods：Arthritis of rat induced by type Ⅱ collage and complete Freund＇s adjuvant was used as rheumatoid arthritis model. The therapeutic effect of Yiqi Tongbi Mixture was observed by investigating CIA rats＇ joint pathological changes, right metapedes radiology and the active expression of VEGF, matrixmetallopmteinase-1,2, 3, 9 （MMPs） in serum.Results： 1. The new vessels in membrana synovialis of Yiqi Tongbi Mixture-treated rats were all conspicuously decreased compared with model group （ P 〈 0.05） ; 2. The radiology change of joint in treated group were improved. 3. The active expression of VEGF and MMPs in serum of model group was obviously stepped up, while the expression of Yiqi Tongbi Mixture-treated group was down-regulated. Conclusion： Yiqi Tongbi Mixture can improve CIA rats＇ joint pathological changes and radiological changes, and to restrain the angiogenesis of CIA rats＇ membrana synovialis by decreasing the expression of VEGF and MMPs in serum.