Caco-2细胞模型及其在中药吸收转运研究中的应用

Caco-2 Cell Model and Its Application in the Absorption and Transportation Research of Chinese Materia Medica

Caco-2细胞模型是一种筛选药物离体口服吸收特性的模型,现已广泛应用于评价药物在小肠的吸收特性和转运机理的研究。作者描述了Caco-2细胞的培养及其细胞单层模型如何建立和验证;探讨了Caco-2细胞用于药物吸收转运研究的机制;对其在中药被动转运、载体介导的主动转运、中药外排机制和吸收过程中药物相互作用等方面的应用进行了详细的阐述;并评价了该细胞用于中药吸收转运机制研究中的作用;最后对该细胞模型可能的应用发展前景提出了展望。

The Caco-2 cell model is an in vitro model for screening drug oral absorption characteristics. It has been used widely for evaluating drug absorption characteristics and transportation mechanism. The authors describ the Caco-2 cells culture and how to establish and verify the cell monolayer model. The mechanism of its application in the drug absorption and transportation study is investigated. The applications for passive transportation of Chinese material medica, vector mediated active transportation, efflux mechanism and the drug-drug interaction in the absorption process are discussed in detail. The efficiency on the absorption and transportation mechanism research of Chinese material medica for the Caco-2 cell is evaluated. Finally, the prospect of the cell model in future application is put forward.