三株丝状真菌分解樟子松凋落物酶活性

Activies of Enzymes During the Litter of Pinus sylvestris var.mongolica Degradation by Three Strains of Mycelial Fungi

该研究以在樟子松凋落物层中高频出现的3株丝状真菌Alternaria sp.、Penicillium sp.和Pestalotiopsis sp.为供试菌株,以樟子松新鲜落叶为作用底物,通过发酵纯培养的方法,测定了底物有机物质质量损失及发酵过程中产生的漆酶(Laccase)、锰过氧化物酶(MnP)、羧甲基纤维素酶(CMCase)和滤纸酶(FPA)酶活性变化,并验证了酶活性与底物降解的关系。结果表明,Alternaria sp.引起底物总有机物质质量损失最大,且产生的漆酶、羧甲基纤维素酶和滤纸酶活性都较高;Peni-cillium sp.产生的锰过氧化物酶活性最高。3株丝状真菌同属于真菌功能群中的木质纤维素分解者。

Three strains of mycelial fungi, Alternaria sp., Penicillium sp. and Pestalotiopsis sp., high- frequently isolated from litter of Pinus sylvestris vat. mongolica were as the research strain,, the current deciduous needles were as the active zymolyte, the method of fermentation was used to assay the mass defect of zymolyte and the activies of enzymes including laccase, manganese peroxidase （MnP）, carboxymethyl cellulase （CMCase） and filter paper activity （FPA） in fermenting process, and try to comfirm the relationship between the activities of enzymes and the mass defect of zymolyte. The results show that the mass defect of zymolyte caused by Alternaria sp. is the highest. Alternaria sp. can produce highest activity of Laccase, CMCase and FPA. Penicillium sp. can produce highest activity of MnE The three strains of mycelial fungi are all belong to lignocellulose-decomposing fungi of the group of functional fungi.