耐盐偶氮染料脱色菌株GYW的筛选及特性

Isolation and Characteristic of an Azo Dye-decolorizing Salt-tolerant Bacteria Strain GYW

从某印染厂排水沟的底泥中分离筛选到1株对偶氮染料具有脱色能力的耐盐菌株GYW,经16SrDNA序列分析,鉴定为盐单胞菌属(Halomonas)中度耐盐菌。实验结果表明,菌株GYW可以耐受10%以上的高盐度,对酸性大红GR和其它偶氮染料具有广谱的脱色能力,处于对数生长期的细胞脱色能力最强。对酸性大红GR的最佳脱色条件为:温度30°C,pH7.5,LB培养基。氯离子对酸性大红GR脱色的抑制作用较强,硫酸盐对脱色影响不大,添加甜菜碱可提高染料的脱色速率,最佳添加量为200mg/L。

A salt-tolerant strain GYW capable of decolorating azo dye was isolated and identified as Halomonas sp. by 16S rDNA. The result showed that the decolorizing salt-tolerant bacteria could survive above the 10% salt concentration and it could decolorize many dyes. The strain had a high decolorizing rate on acid red GR under the condition of pH 7.5, anaerobic 30℃ and 10% NaCl. The ion of Cl^- strong inhibited the decolorization of acid red GR, and the ion of SO4^2- affected little on the decolorization, and the lycine addition with the optimal concentration of 200 mg/L could enhanced the decolorization rate under high NaCl concentration.