猪一般型生殖道放线菌的分离鉴定与系统进化分析

Identification and Phyletic Evolution Analysis of Actinomyces hyovaginalis from Swine

2007年4月,从患有化脓性肺炎、化脓性关节炎和心内膜炎的8周龄病死仔猪内脏器官中分离到一株细菌,对其进行了种属关系鉴定、致病性及药物敏感性等研究。首先从表型特征和生化特性方面进行鉴定,然后应用分子技术对其16SrDNA进行测序分析,最后进行了动物实验和药物敏感性实验。该细菌表型特征和生化特性表明其与生殖道放线杆菌(A.hyovaginalis)非常相似;对16S rDNA测序结果分析发现其与A.hyovaginalisIII群菌株同源性高达99.2%,系统进化分析也表明分离株与A.hyovaginalisIII群菌株亲缘关系最近;对小白鼠具有较强的致病性;对红霉素、庆大霉素和阿米卡星等药物高度敏感。因此,证实分离菌株为有致病性的A.hyovaginalisIII型。

A strain was isolated from internal organ of died porcine about 8 weeks with purulent pneumonia, arthritis, pyogenic arthritis and endocarditis in April 2007. Objectives of the study are to confirm the genus of the strain, pathopoiesis, and drug sensitivity. The mainly study methods： the first, the strain was identified by the phenotype and the characteristics of the biochemistry, sequence 16S rDNA genes of the strain was analyzed by molecular biology technology, finally animal experiment and drug sensitivity testing were done. The results of the phenotype and the characteristics of the biochemistry showed that it is greatly similar to Actinomyces hyovaginalis, 16S rRNA sequence analysis exhibited the homology achieved to 99.2% compared with group Ⅲ strains of Actinomyces hyovaginalis, and the phyletic evolution analysis also indicated that it has mostly relationship with group Ⅲ strains of Actinomyces hyovaginalis. Animal experiment discovered it has highly pathogenicity to Mus musculus albus; Drug sensitivity testing showed that it is hypersensitive to Erycin, Gentamicin and Amikacin. So, the result of the study confirmed that the strain is Actin omyces hyovaginalis Ⅲ with the pathogenicity.