影响杜泊绵羊胚胎移植效果的因素分析

Factors Affecting Embryo Transfer in Dorpor Sheep

系统探讨了在生产条件下杜泊绵羊移植胚胎数量、胚胎发育阶段、受体重复利用、卵巢黄体数量、季节和移植方法对胚胎移植效果的影响。结果表明：移植2枚或3枚胚胎的妊娠率（63．79％、61．90％）显著高于移植1枚胚胎的妊娠率（50．30％）（P〈0．05）；2～8细胞胚胎的移植妊娠率与桑椹胚／囊胚移植妊娠率差异不显著（P〉0．05）；受体重复利用对妊娠率无显著影响（P〉0．05）；受体单侧卵巢上有3个及以上黄体数妊娠率最高，但与单侧卵巢上有1个和2个黄体的妊娠率无显著性差异（P〉0．05）；在秋季进行胚胎移植，妊娠率为59．77％，高于冬季移植的妊娠率（52．94％），但差异不显著（P〉0．05）；常规手术法与腹腔内窥镜法移植相比，妊娠率无显著差异（P〉0．05）.

This study was conducted to investigate the effect of the number of transferred embryos, embryo development stage,repeated utilization of recipients, the number of corpus luteum in single ovary of recipients, season and transplantation methods on pregnancy rate and kidding in dorpor sheep. The results demonstrated that the pregnancy rate of recipients transferred two or three embryos （63.79% or 61.90% ） was significantly higher than that of recipients transferred one embryo （ 50. 30% ） （ P 〈 0. 05 ）. There was no significant difference between the pregnancy rate of recipients transferred 2 - 8 cells and transferred morula/blast embryos. The effect of recipients repeated utilization on pregnancy rate was no significant difference （ P 〉 0. 05 ）. The pregnancy rate of recipients carrying three corpus luteums or even more in single ovary, was the highest, but compared to pregnancy rate of recipients carrying one or two corpus luteums, there was no significant difference （P〉0.05）. The pregnancy rate of recipients was 59.77% in winter, higher than that in autumn （ 52. 94% ）, but no significant difference （ P 〉 0. 05 ） was observed between them. The pregnancy rates of recipients were not significantly different （ P 〉 0.05 ） between surgical operation transfer and laoaroscope transfer.