摘要
建立了猪粪中喹乙醇残留的高效液相色谱检测方法，猪粪经蒸馏水提取震荡后，加入硼砂和硫酸锌等净化剂净化样品，离心，过0．22μm滤膜，HPLC检测，以ZORBAXSB—C18色谱柱为固定相，12％甲醇水溶液为流动相，在波长382nm状态下进行检测。喹乙醇浓度为0．05—10．0μg／ml时与色谱峰面积呈线性关系，绝对系数R^2=0．9999。在0．6—3．0mg／kg浓度添加范围内，回收率为70．07％-83．44％，日内变异系数为1．20％-4．38％，日间变异系数为4．64％-9．62％，样品的检出限为0．4μg／kg。
A HPLC method was developed for determination of olaquindox residues in pig manure to offer a reference for the environmental risk assessment. In the experiment, ZORBAX SB-C18 was used as stationary phase, while the mobile phase comprised of 88.0% distilled water and 12% methanol. Olaquindox residues in pig manure were detected by VWD at wavelength of 382 nm. The calibration curves for olaquindox standard solution was linear （ R^2 = 0. 999 9 ） in a concentration range of 0.05 - 10. 00 μg/ml. The recoveries at olaguindox levels of 0. 6 mg/kg, 1.5 mg/kg ,3.0 mg/kg fortified samples ranged from 70.07% - 83.44% with coefficient of variation of intra-and inter-assay of 1.20% - 4. 38% and 4. 64% -9. 62% respectively. The limit of detection was 0.4 μg/kg.
出处
《江苏农业学报》
CSCD
北大核心
2009年第2期411-415,共5页
Jiangsu Journal of Agricultural Sciences
基金
江苏省自然科学基金项目(BK2006163)
江苏省农业科学院基金项目(028046110608)
