玉米秸秆稀酸预处理的研究

Study on Dilute-acid Pretreatment of Corn Stalk

研究了玉米秸秆稀酸预处理条件对木糖得率和纤维素酶水解性能的影响。在单因素试验的基础上,用正交试验法对稀酸预处理条件进行优化。在固液比1∶10、硫酸质量分数0.75%、温度150℃条件下处理30min,木糖得率最大为85.64%。100g玉米秸秆经稀酸预处理和纤维素酶水解后,可得到最大总糖量49.74g。分析结果表明,木糖得率最大影响因素为酸浓,酶解得率最大影响因素是温度。温度对综合指标的影响极显著,酸浓影响显著,时间影响不显著。预处理破坏了玉米秸秆的纤维素结构。

The effects of conditions of dilute-acid pretreatment of corn stalk on xylose recovery and performance of cellulase were investigated. Orthogonal test was employed to optimize the condition of pretreatment. The results showed that a maximum 85.64 % of xylose recovery was obtained at 150℃, sulfuric acid 0.75 % （mass fraction） and retention time 30 min. The maximal total sugar 49.74 g was obtained from 100 g corn stalk pretreated by dilute-acid under the optimal condition, followed by cellulase hydrolysis. The results showed that in terms of the significance on xylose recovery, sulfuric acid concentration ranked the first factor, temperature the second and reaction time the third. In terms of the significance on enzymatic digestibility, however, temperature ranked the first factor, sulfuric acid concentration the second and reaction time the third. The comprehensive index was very significantly influenced by temperature and significantly influenced by sulfuric acid concentration. Pretreatment destroyed the fibrous structure of corn stalk significantly.