均匀设计法优选大孔树脂纯化七叶莲总皂苷工艺研究

Study on Optimum Purification Process for Total Saponins of Schefflera arboricola Hayata by Macro-porous Absorptive Resins Using Uniform Design

以总皂苷比吸附量和解吸率为指标,通过静态和动态吸附解吸附试验,从9种树脂中筛选出AB-8型大孔吸附树脂;以总皂苷收率和总皂苷纯度为指标,通过单因素试验考察了最佳洗脱剂浓度,并利用均匀设计法对大孔树脂富集纯化的工艺进行了优化。结果表明:以70%乙醇为洗脱剂,以总皂苷收率为指标,优选的工艺参数为:上样质量浓度0.976g/L、上样流速2.2mL/min、洗脱剂用量3.0BV(1BV=80mL,下同)、洗脱流速3.6mL/min,其总皂苷收率62.17%,总皂苷纯度41.22%;以总皂苷纯度为指标,优化的工艺参数为:上样质量浓度0.976g/L、上样流速2.8mL/min、洗脱剂用量0.6BV、洗脱流速0.8mL/min,其总皂苷收率41.25%,总皂苷纯度49.79%。

In order to establish a purification process for total saponins of Schefflera arboricola Hayata, nine kinds of macro-porous absorptive resins were tested and AB-8 was selected for its excellent absorptive and desorptive properties. Then the process parameters were optimized by single factor tests and uniform design, and statistical methods. Using recovery rate as index, the optimum conditions were obtained as following： sample mass concentration 0.976 g/L, feed rate 2.2 mL/min, elution rate 3.6 mL/min, eluant amount 3.0 BV（ 1 BV = 80 mL） ; recovery rate reached 62.17 % and purity was 41.22 %. Using purity as index, the optimum condtions were obtained as following： sample mass concentration 0.976 g/L, feed rate 2.8 mL/min, elution flow rate 0.8 mL/min, eluant amount 0.6 BV; purity reached 49.79 % and recovery rate was 41.25 %.