^(131)I标记花生凝集素的制备及其受体亲合活性

The Preparation of 131 IPeanut Agglutinin and Its Affinitive Activity

本文报道１３１碘－花生凝集素（１３１Ｉ－ＰＮＡ）的制备方法。采用Ｉｏｄｏｇｅｎ法进行放射性碘化标记，标记率为６４％～７１％，平均标记率６７．９％；放化纯度为８７％～９８％。经柱层析后得到纯品，通过纸层析鉴定为单一放射性峰，用自身取代法测定其比放射性为２０９～３１４ｋｂｑ／μｇ。它与ＰＮＡ受体结合，具有亲合力高及特异性强的生物活性。

An Iodogen method was used for the radioiodization of peanut agglutinin.The iodination rate was 64%～71%,and radiochemical purity was 87%～98%.The purified product by column chromatography showed a single radioactive peak when examined by paper chromatography.Its specific activity,measured by selfdisplacement method,was 209～314 kbq/μg.The binding of this compound with PNA receptor has high affinity and specificity.