HPLC测定复方庆大霉素膜中醋酸地塞米松的含量

Determination of dexamethasone acetate in compound gentamycin pellicles by HPLC

目的建立用高效液相色谱法(HPLC)对复方庆大霉素膜中醋酸地塞米松进行含量测定的方法。方法采用Synergi 4u Hydro-rp 80A(250mm×4.60mm,4 micron)色谱柱,以甲醇-水-三乙胺(695∶300∶5)为流动相,检测波长为240nm,流速为1.0mL.min-1。结果醋酸地塞米松的线性范围为12～96μg.mL-1(r=0.9999,n=5);平均回收率为98.8%(RSD=0.83%,n=6)。结论本方法结果准确,重现性好,适用于该复方制剂中醋酸地塞米松的含量测定。

OBJECTIVE To establish a method for the content determination of dexamethasone acetate in compound gentamycin pellicles by HPLC. METHODS Synergi 4u Hydro-rp 80A（250mm × 4.60mm, 4 micron） column was used, methanol-water-triethylamine（695： 300： 5） was used as mobile phase, the detection wavelength was at 240nm, the flow rate was 1.0mL·min- 1. RESULTS The linear range of dexamethasone acetate was 12 96μg· mL- 1 （r = 0. 9999, n = 5 ） ; the average recovery rate was 98.8 96 （ RSD = 0.83 96, n = 6 ）. CONCLUSION The method is highly repeatable and accurate, which can be used for determination of dexamethasone acetate in compound gentamycin pellicles.