巢式PCR技术检测泌尿生殖道标本中沙眼衣原体的研究

Direct Detection of Chlamydia trachomatis in Clinical Samples of Genital Infection by Using a Nested PCR.

采用巢式ＰＣＲ检测５１３例泌尿生殖道感染病人标本中的沙眼衣原体（ＣＴ）。结果显示：经主要外膜蛋白（ＭＯＭＰ）基因引物第一次扩增阳性率为９．９４％（５１例），第二次扩增后阳性率为２１．８０％（１１２例）。表明应用巢式ＰＣＲ检测沙眼衣原体ＭＯＭＰ基因具有敏感。

The authors reported the detection of CTMajor Membrane Protein MOMP gene in 513 clinical samples of genital infection by PCR.9.94%(51) was positive.In the second round of PCR,21.8%(112) was positive.The results suggested that it is a sensitive and specific way to detect CTMOMP gene by using a nested PCR.