镉对大鼠肝细胞DNA损伤作用的研究

Effect of Cadmium on DNA Damaging in Rat Liver Cells

应用单细胞凝胶电泳研究氯化镉对大鼠肝细胞ＤＮＡ的损伤作用。结果表明，８．７５μｍｏｌ／Ｌ、１７．５μｍｏｌ／Ｌ、３５μｍｏｌ／Ｌ氯化镉可以引起肝细胞ＤＮＡ单链断裂，拖尾的慧星细胞分别达到７８％、９０％、９８％，并存在着明显的剂量效应关系，相关系数０．９９３３。本研究提示，氯化镉可以引起离体肝细胞ＤＮＡ损伤。

Using single cell gelelectrophoresis or Comet assay,this article presents the effect of cadmium chloride on DNA single strand breaks in rat liver cells.The results show that at the concentration of 8 75uM,17 5uM,35uM,cadmium chloride can induce DNA damage and result in Comet cells.The number of Comet cells is more than that of control,P<0 01,and the doseeffect relationship is obvious.The research infers that at certain concentration,cadmium can induce DNA damage and has toxic effect on rat liver cells.