不同缺血后处理方案抗大鼠肾缺血-再灌注损伤的作用

Protective Effects of Ischemic Postconditioning on Renal Ischemic Reperfusion Injury in Rats

目的探讨不同缺血后处理方案抗大鼠肾缺血-再灌注损伤的作用.方法在大鼠右肾切除,左肾I45 min R24 h缺血-再灌注模型上,分别给予:(1)夹闭(缺血)10 s/松夹(再灌注)10 s 6个循环;(2)20 s/20 s 3个循环;(3)30 s/30 s 3个循环;(4)60 s/60 s 3个循环;(5)10 s/30 s 3个循环,(6)30 s/20 s 3个循环的缺血后处理方案干预后,观测HE染色肾组织病理形态学变化和血肌酐和血尿素氮水平.结果6种不同缺血后处理方案均能减轻肾组织病理形态学损伤和降低血肌酐和血尿素氮水平.结论不同缺血后处理方案均可减轻肾脏缺血-再灌注损伤,以6个循环10 s/10 s方案最佳.

Objective To investigate the protective effects of different schemes of ischemic postconditioning on renal ischemic reperfusion injury in rats. Methods After right nephrectomy, rats were subjected to 45 min of left renal ischemia followed by 24 h reperfusion, and different schemes of ischemie postconditioning were given：six cycles of 10 s reperfusion/10 s occlusion, three cycles of 20 s reperfusion/20 s occlusion, three cycles of 30 s reperfusion/30 s occlusion, three cycles of 60 s reperfusion/60 s occlusion, three cycles of 10 s reperfusion/30 s occlusion, three cycles of 30 s reperfusion/10 s occlusion, followed by 24 h reperfusion. Renal function was evaluated by the levels of serum creatine and urea nitrogen; the renal pathologic change was observed by HE staining. Results Our data showed that ischemic postconditioning decreased the levels of serum creatine and urea nitrogen and improved the renal pathologic change. Conclusions Different schemes of ischemic posteonditioning may improve renal function. The optimical postconditioning scheme is six cycles of 10 s reperfusion/10 s occlusion.