猪卵母细胞体外成熟与孤雌激活的研究

In Vitro Maturation and Parthenogenetic Activation of Porcine Oocytes

研究了生长激素、胰岛素对猪卵母细胞体外成熟的影响,比较了电激活、乙醇激活、A23187激活3种激活方法对成熟卵母细胞孤雌激活的影响。试验结果表明:在mNCSU-23基础液中添加Insulin对卵母细胞体外成熟呈现双重效应。在mNCSU-23基础液中添加5μg/mLInsulin和8μg/mLInsu-lin组的卵母细胞成熟率(66.25±1.34)%、(60.64±1.64)%显著高于对照组及0.5、2、10μg/mL组;添加5μg/mL Insulin组的卵裂率(61.63±2.92)%显著高于对照组及0.5、2、8、10μg/mL组(P<0.05);0.15μg/mL STH与2μg/mL Insulin联合添加组成熟率最高(79.37±2.10)%,同其它组相比,差异显著(P<0.05),在此试验中为猪卵母细胞体外成熟的最佳条件。电激活比乙醇激活、A23187激活有更高的卵裂率(67.75±1.03)%和桑椹胚率(13.52±2.05)%,且差异显著(P<0.05),最佳激活参数为130V/mm、80μs、一次脉冲激活。

Effect of somatotropin and insulin on in vitro maturation of porcine oocytes was studied. The best method and parameter were selected by comparing electrical, A23187 and ethanol activation on porcine ooeytes. The results showed that adding insulin in mNCSU -23 basic liquid had double effects on ooeyte maturation. The maturation rate gotten by adding 5 μg/mL and 8 μg/mL insulin were （66.25±1.34 ） % and （60.64±1.64 ） %, respectively, markedly higher than those of comparison groups as well as those of groups that 0.5, 2 and 10μg/mL insulin were added. The cleavage rate in the group that 5μg/mL insulin was added was （61.63±2.92）%, notably higher than that of the comparison group as well as those of groups that 0.5, 2, 8 and 10μg/mL insulin were added （P 〈0.05 ）. The maturation rate （79.37 ± 2. 10）% was the highest when 0. 15μg/mL somatotropin and 2μg/mL insulin were added, thus coming out to be the best conditions for porcine ooeytes to achieve in vitro maturation. Among the three kinds of activation methods, electrical activation was the best with （67.75±1.03） % ooeytes cleavage rate and （13.52±2.05）% morulae rate. The best parameters for electrical activation was 130 V/ mm and 80μs.