电针对脑缺血再灌注大鼠外周血内源性内皮祖细胞的作用

Effect of Electroacupuncture on Plasma Endogenous Endothelial Progenitor Cell Counts in Cerebral Ischemia-reperfusion Rats

目的:初步探讨电针对脑缺血后内源性内皮祖细胞(EPCs)及其相关因子的影响。方法:72只SD大鼠随机分为正常组、假手术组、模型组、针刺组,各组分24、48、72h3个时间点,每个时间点6只。大脑中动脉闭塞法制造脑缺血再灌注模型。电针刺激大鼠单侧"足三里""曲池",针刺30min,每日1次。使用流式细胞术检测EPCs的数量,ELISA法检测血清中内皮生长因子(VEGF)含量,分光光度法检测总一氧化氮合酶(TNOS)、诱导型一氧化氮合酶(iNOS)活力。结果:模型组脑缺血再灌注后24h外周血EPCs数量和血清TNOS、iNOS活力都明显升高(P<0.01,P<0.05);48h时,针刺组EPCs数量升高,与其它各组相比差异有统计学意义(P<0.05,P<0.01);72h时,针刺组EPCs数量较模型组降低(P<0.01)。针刺组在各时间点血清中VEGF含量都高于其它各组(P<0.01,P<0.05)。结论:脑缺血再灌注损伤后外周血EPCs数量和血清iNOS活力增加。电针能够影响脑缺再灌注损伤大鼠内源性EPCs,该作用可能与VEGF表达上调有关。

Objective To observe the effect of electroacupuncture（EA）on peripheral blood endothelial progenitor cell（EPC）counts,vascular endothelial growth factor（VEGF）level and total nitric oxide synthase（TNOS）and inducible nitric oxide synthase（iNOS）activity in cerebral ischemia-reperfusion injury（CI/RI）rats.Methods A total of 72 male rats were randomly and evenly assigned to normal control,sham-operation（sham）,model and EA groups which were further divided into 24 h,48 h and 72 h subgroups,with 6 cases in each.Acute focal cerebral ischemia model was established by occlusion of the middle cerebral artery（MCAO,120 min）and reperfusion.EA（2/15 Hz,1 mA）was applied to ＂Quchi＂（LI 11）and ＂Zusanli＂（ST 36）for 30 min,once daily.Peripheral blood was collected from abdominal aorta for detecting EPC count by using flow cytometry,serum VEGF level by using enzyme-linked immunosorbent assay（ELISA）,and serum TNOS and iNOS activity by spectrophotometry,respectively.Results Compared with the corresponding normal control subgroups,blood EPC counts,serum TNOS and iNOS activity and serum VEGF content at 24 h,iNOS activity and VEGF level at 48 h,and EPCs and iNOS at 72 h in model subgroups all increased significantly（P〈0.01,P〈0.05）.In comparison with the corresponding model subgroups,EPC count at 24 h and 72 h,and TNOS activity at 24 h in EA subgroups decreased considerably（P〈0.01,P〈0.05）;while EPC and VEGF levels at 48 h in EA subgroup increased evidently（P〈0.05,P〈0.01）.No significant differences were found among normal,sham,model and EA subgroups in serum TNOS activity at 48 h and 72 h（P〉0.05）.Conclusion EA of LI 11 and ST 36 can suppress CI/RI induced increase of blood EPC count and serum TNOS activity,and upregulate serum VEGF level,which may contribute to its effect in relieving CI/RI.