绞股蓝总皂甙对肝脏四氯化碳损伤保护作用的实验研究

Experimental study on protective effect of Gypenosides against carbon tetrachloride─induced hepatic damages

给慢性四氯化碳（ＣＣ１＿４）肝损害小鼠灌喂绞股蓝总皂甙（ＧＰｓ），剂量为每次７５ｍｇ／ｋｇ或１５０ｍｇ／ｋｇ（每周５次，共３周），均能明显减轻肝组织损害，与未治疗组比较差异有显著意义（Ｐ＜０．０５和Ｐ＜０．０１）。用ＧＰｓ治疗的两组小鼠血清丙氨酸转氨酶（ＡＬＴ）活性均较未治疗组降低，血清白蛋白含量和Ａ／Ｇ比值较未治疗组升高。在原代培养大鼠肝细胞损伤模型上，预先用浓度为１０ｍｇ／Ｌ和４０ｍｇ／Ｌ的ＧＰｓ处理大鼠肝细胞，肝细胞ＤＮＡ合成速率分别从ＣＣ１＿４损伤组的３１．９％升高到６７．６％（Ｐ＜０．０５）和８２．４％（Ｐ＜０．０１）；肝细胞培养液中ＡＬＴ活性均明显低于损伤组（Ｐ＜０．０１）。表明ＧＰｓ能减轻ＣＣ１＿４诱导的小鼠慢性肝损害和离体大鼠肝细胞损伤，改善肝脏合成白蛋白的功能，保护肝细胞ＤＮＡ合成。

To evaluate the hepatoprotective effect of Gypenosides ( GPs ) , the models of carbon tetrachloride (CC1_4) -induced chronic hepatic damage in mice and hepatocellular injury in primary cultured rat hepatocytes were employed . The results showed that after administering GPs to mice , either 75 mg/kg or 150 mg/kg a day , for 3 weeks , the liven damaged by CC1_4 were improved obvious- ly . The alanine transaminace ( ALT) activity decreased and albumin and A/G ratio increased in serum of GPs treated mice in comparison with controls . Meanwhile , in rat hepatocytes pretreated with GPa , the DNA synthesis rate was enhanced and the ALT activity was re- duced dose-dependently , as compared with the hepatocytes untreated with GPs . It is suggested that GPs can protect against CC1_4-in- duced damage in both mouse liver and cultured rat hepatocytes , owing to the inhibition of lipid peroxidation by GPs .