三唑类衍生物对肝癌细胞株BEL-7402增殖的影响

Effects of Benzotriazole on Proliferation of Hepatocarcinoma BEL-7402 Cancer Cells

通过体外药敏性实验,对几种三唑类衍生物抗肿瘤活性进行了研究,从Cf、Db、Cc、Cg、Dc 5种三唑类化合物中筛选出Db。实验表明,Db对肝癌细胞株BEL-7402增殖有明显的抑制作用,抑制率呈浓度和时间依赖性。进一步实验证明,Db能够通过引发细胞产生自由基、线粒体膜电位下降等途径诱导细胞凋亡。

Benzotriazole compounds have been found to show extensive biological activities. Many of them have been developed and used as fungicides, plant growth regulators and medicine. In the present study, we find five new bioactive benzotriazole derivatives Cf,Db,Cc,Cg,Dc, of which one compound Db was selected. It was demonstrated that Db could significantly inhibit proliferation of cultured hepatoearcinoma BEL-7402 cancer cells in a dose- and time dependent manner. Treatment with Db resulted in the genera- tion of reactive oxygen species （ROS）, disruption of mitochondrial membrane potential （△ψm） , DNA fragmentation, and apoptosis in BEL-7402 cells.