牛血小板衍化生长因子的生物学活性研究——对BALB/c 3T3细胞DNA合成的影响

Mitogenic effects of bovine platelet-derived growth factor on BALB/c 3T3 cells

目的：检测牛血小板衍化生长因子的致丝裂活性。方法：以ＢＡＬＢ／ｃ３Ｔ３细胞为靶细胞，利用３Ｈ－ＴｄＲ掺入合成ＤＮＡ的方法。结果：５ｎｇ／ｍｌ牛血小板衍化生长因子可明显促进处于静止状态的ＢＡＬＢ／ｃ３Ｔ３细胞ＤＮＡ合成，３０ｎｇ／ｍｌ浓度使细胞ＤＮＡ合成达最高峰，并在２４ｈ最为显著；热处理对其致丝裂活性无影响；而经２－巯基乙醇处理后其活性基本丧失。结论：牛血小板衍化生长因子具有良好的致丝裂活性，耐热，２－巯基乙醇可使其丧失活性。

Aim: The mitogenic activity of bovine platelet-derived growth factor was detected. Methods: 3H-TdR incorporation in BALB/c 3T3 cells. Results: Bovine platelet-derived growth factor stimulated DNA synthesis of quiescent BALB/c 3T3 cells in a dose-dependent manner over a concentration range of 5ng/ml to 40ng/ml, with a maximal response at a concentration of 30ng/ml at 24h, The factor was stable to heat(100℃). The mitogenic activitg of it was destroyed with 2-mercaptoethanol. Conclusions: Bovine platelet-derived growth factor had strong mitogenic activiy and heat-resistant, but lost the mitogenic activity with 2-mercaptoethaol.