丙戊酸钠抑制肺癌细胞株A549增殖的研究

Proliferation inhibition of sodium valproate on lung cancer cell line A549

目的研究丙戊酸钠（sodium valproate,VPA）对肺癌细胞株A549细胞增殖的影响。方法体外不同浓度VPA不同作用时间处理人肺癌细胞株A549,倒置显微镜观察细胞形态变化,MTT比色法检测细胞增殖,流式细胞技术分析细胞周期与细胞凋亡的改变。结果2.0 mmol/L VPA作用72 h细胞形态明显改变、细胞数明显减少。1.0mmol/L VPA作用72 h后,细胞增殖被抑制,抑制率为（15.8±2.8）%,与同期对照组相比,差异具有统计学意义（P〈0.05）;提高处理浓度或延长作用时间,抑制作用有加强趋势。1.0 mmol/L VPA作用72 h后可改变细胞周期分布,G1期细胞比例为（72.2±3.4）%,与同期对照组相比差异显著;2.0 mmol/LVPA处理24～72 h后G1细胞比例由（64.5±3.7）%增加至（79.8±4.4）%,而S期细胞由（30.7±5.17）%降低至（14.2±3.37）%;提高处理浓度或延长作用时间均使G1期细胞比例增高,S期细胞比例减少。2 mmol/L VPA作用24～72 h可提高细胞凋亡率,由（7.30±1.87）%增加到（19.85±2.40）%。结论VPA可抑制肺癌A549细胞生长,改变细胞周期分布、促进细胞凋亡。

Objective To investigate the effect of sodium valproate（VPA） on proliferation of lung cancer cell line A549.Methods A549 cells were treated with different concentration of VPA for various time.Cell morphology of A549 treated with VPA was observed by invert microscope in vitro.Cell proliferation was detected by MTT assay.Apoptosis and cell cycle were analyzed by flow cytometry.Results The population of A549 cells decreased and there was an obvious change in shape when A549 cells were treated with 2.0 mmol/L VPA for 72 hours.The proliferation of A549 cells was inhibited by treating with 1 mmol/L VPA for 72 hours,and the proportion of G1 cell was（72.2±3.4）%.There was significant difference as compared with the control group（P〈0.05）.Cell cycle was changed by treating with 2 mmol/L VPA for 24 to 72 hours.The cells in G1 phase increased from（64.5±3.7）% to（79.8±4.4）% and the cells in S phase decreased from（30.7±5.17）% to（14.2±3.37）%.The ratio of G1 phase cells increased remarkably under treatment with higher concentration and/or longer time of VPA,and the ratio in S phase cells decreased significantly.Cell apoptosis increased from（7.30±1.87）% to（19.85±2.40）% after treated with 2 mmol/L VPA for 24 to72 hours.Conclusion VPA can significantly suppress the cell proliferation,change cell cycle and induce cell apoptosis.