托瑞米芬治疗乳腺增生症的实验研究

Effect of Toremifene on hyperplasia of mammary gland

目的观察托瑞米芬对大鼠乳腺增生及性激素水平的影响。方法用苯甲酸雌二醇每日注射复制动物模型,放免法测定性激素水平,免疫组织化学法分析不同组标本血管内皮细胞生长因子(vascular endothelial growthFactor,VEGF)、微血管密度(microvessel density,MVD)、碱性成纤维生长因子(basic fibroblast growth factor,bFGF)的变化。结果托瑞米芬治疗大鼠乳腺增生总有效率为100%,小叶腺泡数、导管上皮层数、腺体厚度明显变薄(P<0.05),有调节性激素水平的作用,有明显的量效关系。而且托瑞米芬可明显降低大鼠乳腺增生标本VEGF、bFGF、MVD的表达,有明显的量效关系。结论托瑞米芬能不同程度抑制大鼠乳腺增生,明显促进腺体退化,并能调整机体内源性激素水平,还可以降低VEGF、bFGF、MVD的表达,改善乳腺慢性增生的病变过程,达到治疗的目的。

Objective To observe the effect of Toremifene（TOR） on hyperplasia of mammary gland and the blood sex hormones of rat.Methods Benovocylin was injected every day to duplicate animal model.Levels of sex hormone of blood were measured by means of radio-immunoassay.Expressions of vascular endothelial growth factor（VEGF）,basic fibroblast growth factor（bFGF） and micro vascular density（MVD） were detected by immunohistochemistry.Results The total efficiency rate of TOR on hyperplasia of mammary gland was 100%.TOR could decrease the number of leaflet acinus,duct epithelial layer and weight of mammary glands.It had a dose-effect relationship in some extent（P〈0.05）.TOR could decrease the expressions of VEGF,bFGF and MVD.There was significant dose-effect relationship among different dose of TOR on its effect of the expressions of VEGF,bFGF,MVD.Conclusion TOR can inhibit hyperplasia of mammary glands of rat.TOR can adjust the endogenous sex hormone level,delay the development of chronic serious hyperplasia of mammary gland.TOR can decrease the expressions of VEGF,bFGF,MVD in rats.