巨噬细胞集落刺激因子对小鼠腹腔巨噬细胞抗氧化能力的影响

Effect of Macrophage Colony Stimulat-ing Factor on the Antioxidative Capacityof Murine Peritoneal Macrophages

越来越多的实验结果证实巨噬细胞集落刺激因子在动脉粥样硬化发生过程中的重要作用，为了探讨巨噬细胞集落刺激因子和活性氧与动脉粥样硬化之间的相互关系，观察了巨噬细胞集落刺激因子对培养的小鼠腹腔巨噬细胞抗氧化酶活性的影响。结果发现巨噬细胞集落刺激因子能使小鼠腹腔巨噬细胞谷胱甘肽过氧化物酶活性提高３２％，超氧化物歧化酶的活性提高１２０％，并减轻叔丁基氢过氧化物促巨噬细胞源性泡沫细胞形成，以及提高巨噬细胞的生存数。此结果提示巨噬细胞集落刺激因子提高杭氧化酶活性的作用可能是其虽增加对氧化修饰低密度脂蛋白和胆固醇的摄入却能阻止泡沫细胞形成及动脉粥样硬化发生发展的原因之一。

ABSTRACT Aim The effects of macrophage colony stimulatingfactor (0CSF) on the antioxidative capacity and foamcell formation of murine peritoneal macrophages wereobserved.Methods MCSF was in the conditional cultural liquidsof L929 cell line. Superoxide dismutase(SOD) activi-ty was detected by Pyroyallol method, and seleniumdependent glutathione peroxidase (SeGSHPx) activitywas detected by DTNB method, the survival numberof murine macrophages was detected by MTT method.Results MCSF could enhance SeGSHPx and SOD ac-tivities , moreover , it could prevent foam cell formationcaused by active oxygen and elevate the survival num-ber of murine macrophages.Conclusion The enhancement of antioxidase activitiesby MCSF may be one of the reasons that MCSF canprevents foam cell formation and retard the progressionof atherosclerosis.KEY WORDS Macrophage colony stimulating factor;Seleniumdependent glutathione peroxidase: Super-oxide dismutase; Foam cell formation; Atheros-clerosis