摘要
通过观察神经肽Ｙ和血管活性肠肽对培养血管内皮细胞功能的影响，探讨神经体液因素在动脉粥样硬化发生中的作用。培养的内皮细胞来自小公猪主动脉，内皮源性舒张因子一一氧化氮含量采用酶标比色法测定亚硝酸盐来表示，内皮素采用放射免疫法测定。结果发现，神经肽Ｙ可刺激内皮细胞分泌释放内皮源性舒张因子和内皮素（Ｐ＜０．０５）；但对内皮素分泌释放的刺激作用明显强于内皮源性舒张因子，使神经肽Ｙ表现出血管收缩的综合效应。血管活性肠肽则抑制内皮细胞分泌释放内皮素（Ｐ＜０．０５），降低血管对缩血管物质的敏感性。上述研究结果说明，神经体液因素中神经肽Ｙ和血管活性肠肽等肽类物质可影响血管内皮细胞的功能而参与动脉粥样硬化的发生。
ABSTRACTAim Using cultured porcine endothelial cells withsensitive quantitative methods, this study aims to in-vestigate the regulating effect of neuropeptide Y (NPY)and vasoactive intestinal peptide (VIP) on endothelialfunctions. The work should be optional for betterunderstanding the possible role of neurocrine factors inatherogenesis.Methods A specific and sensitive diazotization assayand radioimmunoreactive analysis were used respec-tively to measure the release of nitric oxide (NO) andendothelin (ET) from porcine aorta endothelial cellsgrown in culture.Results NPY and VIP could affect the release ofNO and ET from cultured endothelial cells. Underthe influence of NPY, the content of NO2 and ET inendothelium-conditioned medium were increased withNPY concentration (P<0. 053 , but the increase of ETwas larger than NO in NPY group. In VIP group,the content of ET in endothelium-conditioned medium was decreased with VIP concentration (P＜0. 05 ).Conclusion The first , NPY may stimulate culturedporcine aorta endothelial cells to release the NO andET, but the increase of ET is more than NO. Thesecond, VIP may inhibit the secretion of ET from cul-tured endothelial cells.KEY WORDS Neuropeptide Y; Vasoactive in-testinal peptide; Vasoactive substances; Atheroge-nesis
出处
《中国动脉硬化杂志》
CAS
CSCD
1998年第1期42-45,共4页
Chinese Journal of Arteriosclerosis
