胃癌中癌基因ras c-mycm RNA表达的临床意义

Oncogene ras, cmyc mRNA expression in gastric carcinoma and its clinical significance

目的探索癌基因ｒａｓ和ｃｍｙｃｍＲＮＡ在胃癌发生中单独和协同作用关系，选择胃癌的早期诊断依据．方法应用原位分子杂交（ＩＳＨ）技术，检测８８例胃癌组织中Ｈｒａｓ，Ｋｒａｓ，ＣｍｙｃｍＲＮＡ的表达，结合临床资料综合分析．结果Ｋｒａｓ，Ｈｒａｓ，ＣｍｙｃｍＲＮＡ在肿瘤区的阳性表达分别为７８４％，７０５％和５８０％，在癌旁区的阳性表达分别为２８４％，２７３％和２０５％，呈梯度下降趋势，３种癌基因的表达与胃癌的临床分型、组织学分类及有无淋巴结转移无关，ＣｍｙｃｍＲＮＡ的表达与患者生存期关系密切（Ｐ＜００５）．结论Ｋｒａｓ，Ｈｒａｓ，ＣｍｙｃｍＲＮＡ的表达可作为胃癌早期诊断的参考依据．

IM To study the independent and combined effects of ras and cmyc mRNA expression in the tumor occurrence and to seek evidence for early diagnosis of gastric carcinoma.METHODS In situ hybridization histochemistry (ISH) was used to examine the expression of Hras, Kras, Cmyc mRNA in 88 cases of gastric carcinoma tissue, and an analysis with clinical data was made.RESULTS Kras, Hras and Cmyc mRNA expression rate in the tumor tissues was 784%, 705% and 580%, and it was 284%,273% and 205% in the neighboring regions of the tumor respectively, with a tendency of stepdown. The oncogene expression was not related to clinical and pathological type and the metastasis to lymph nodes. Cmyc mRNA expression was closely related to the survival time of patients (P<005).CONCLUSION Kras, Hras and Cmyc mRNA expression is a valuable reference for the early diagnosis of gastric carcinoma.