大鼠实验性肝硬变腹水形成与TNFα的关系

Relationship between ascites formation and TNFα in experimental rat liver cirrhosis

目的研究大鼠实验性肝硬变晚期腹水形成与ＴＮＦα及肠源性内毒素血症的关系，探讨肝硬变腹水形成的机制．方法以大鼠为实验对象，分为正常对照组（ｎ＝８）、肝硬变对照组（ｎ＝１２）和肝硬变伴腹水组（ｎ＝１８）．实验组采用复合因子复制肝硬变动物模型．各组均测定腹主动脉血中ＴＮＦα和内毒素水平，肝硬变组还测定了腹水量．结果随着肝硬变形成，大鼠血中ＴＮＦα水平（μｇ／Ｌ）增高，各组含量分别为９０８±１０７和９５８±１２８（肝硬变组和肝硬变伴腹水组）．相关分析表明肝硬变组腹水量与血中ＴＮＦα成正相关（ｒ＝０８６，Ｐ＜００５，内毒素水平增高，并与ＴＮＦα的浓度成正相关（ｒ＝０７５，Ｐ＜００５）．结论肝硬变腹水形成与血中ＴＮＦα升高相关，而ＴＮＦα的升高则源于肠源性内毒素血症的形成．

IM To investigate the relationship between TNFα and ascites of liver cirrhotic rats.METHODS TNFα and endotoxin in the aorta plasma were detected in experimental liver cirrhotic rats (n=12) and cirrhotic rats with ascites group (n=8). And the quantities of ascites in the cirrhotic rats with ascites were also determined.RESULTS Both of cirrhosis group and group of cirrhosis with ascites had increased TNFα and endotoxin. There was a positive correlation between TNFα and endotoxin (r=075, P<005) in the cirrhosis ascites group, and a positive correlation between TNFα and quantities of ascites (r=086,P<005).CONCLUSION Ascites formation in cirrhotic rats is correlated with increased TNFα induced by intestinal endotoxemia.