摘要
为了探讨扶正化瘀方抗肝纤维化的作用机制。以体外培养的ＮＩＨ／３Ｔ３细胞作为肝脏成纤维细胞的替代模型，用扶正化瘀方药物血清，以１０％的浓度被添加到培养液中，通过［＾３Ｈ］ＴｄＲ掺入法和免疫细胞化学，观察药物血清对３Ｔ３细胞［＾３Ｈ］－ＴｄＲ掺入量和胶原生成的影响。结果表明药物血清组［＾３Ｈ］－ＴｄＲ掺入量明显少于正常大鼠血清组。药物血清组Ⅰ、Ⅲ、Ⅳ型胶原量均明显低于正常大鼠血清组，其中Ⅰ。
In order to search the mechanism of FZHYF' s inhibiting the progress of liver fibrosis. By method of [3H] - TdR incorporation and immunocytochemistry we observed the effects of the FZHYF - treated rat sera, which were obtained from the rats administrated orally by FZHYF, on the proliferation and collagen synthesis of NIH/3T3 cell which is a putative model in vitro instead of fibrobalsts in liver in vivo. The re- suits showed that FZHYF - treated rat sera could significantly inhibit [3H] - TdR incorporation of N1H/ 3T3 cell. The FZHYF -treated rat sera could significantly decreased the collagen ( I -, UK IV ) synthesis of 3T3 in vitro. Particularly the collagen( I > HI )were obviously decreased. These indicated that FZHYF - treated rat sera significantly inhibited proliferation >and collagen synthesis of NIH/3T3 cell in vitro.
出处
《中国中医药科技》
CAS
1998年第2期75-77,共3页
Chinese Journal of Traditional Medical Science and Technology
基金
国家自然科学基金资助项目 NO39570889
