H9c2细胞株在心肌缺氧／复氧实验中的应用

The application of H9c2 cell line in the experiment of hypoxia/reoxygenation

目的本实验对H9e2细胞株进行缺氧／复氧，旨在引进一种操作、培养简便，可传代且功能与分离培养的原代细胞相似的大鼠心肌细胞株。方法培养的H9c2细胞随机均匀分为常氧对照组（NC组，6只）和缺氧／复氧组（H／R组，6只）。细胞置于含95％N2，5％CO2的三气培养箱内缺氧3h，置于含95％空气、5％CO2的CO2培养箱中进行复氧培养。台盼蓝染色记数细胞，MTT法检测细胞存活率，检测培养基上清中乳酸脱氢酶（LDH）含量反映细胞损伤程度，并与在分离培养的乳鼠原代心肌细胞上进行的研究做对比。结果与常氧组相比，H9c2细胞缺氧／复氧处理后，存活率明显降低[（68．65±7．55）％比（91．95±9．13）％，P〈0．01]，LDH漏出量增加[（132．38±24．07）U／L比（34．69±9．86）U／L，P〈0．01]。原代乳鼠心肌细胞缺氧／复氧处理后，LDH漏出量增加[（428．62±56．65）U／L比（116．02±23．01）U／L，P〈0．01]。结论H9c2细胞株具有与原代培养乳鼠心肌细胞相似的功能特性，易于分离培养，并可传代，在缺氧／复氧等实验中具有广阔的应用前景。

Objective To observe the effects of hypoxia/reoxygenation on H9c2 rat cardiomyoblast cells derived from the embryonic rat heart. Methods Cultured H9c2 rat cardiomyoblast cells were randomly divided into normal control （NC,n=6） group and hypoxia/reoxygenation （H/R） group. In the period of hypoxia, H9c2 cells were cultured at 37 ℃, 95% N2, 5% CO2 incubator, while in the period of reoxygenation the cells were in a humidified atmosphere of 95% air and 5% CO2 at 37 ℃. Results Compared with NC,the myocardial survival was significantly decreased in H/R group （68.65±7.55）% vs（91.95±9.13）%,P〈0.01 ] and LDH leakage increased significant [ （132.38±24.07）U/L vs（34.69±9.86）U/L,P〈0.011. Conclusion H9c2 rat cardiornyoblast cells have the similar characteristics of function with the primary cultured cardiomyocytes of rat and are easy to be cultured and generated which can be used in more fields [ （68.65±5.42）% vs （91.95±7.13）%, P〈0.01 ].