黄芪总黄酮对心肌细胞内游离钙离子浓度的影响

The effects of total flavonoids of ast ragalus（ TFA ） on the intracellular free calcium concentration of cultured ventricular myocytes

目的研究黄芪总黄酮（Total flavonoids of Ast ragalus，TFA）对体外培养的乳鼠心肌细胞内游离钙离子浓度的作用。方法实验应用钙离子荧光指示剂Fluo-3AM负载原代培养的乳鼠心肌细胞，通过激光共聚焦扫描显微镜上机检测，记录其荧光强度变化，同时观察黄芪总黄酮对乳鼠心肌细胞及化学处理因素H2O2损伤大鼠心肌细胞的荧光强度的作用。结果①H2O2损伤的乳鼠心肌细胞游离钙离子[Ca^2＋]i平均荧光强度值为（1346．89±65．36）nmol／L，与对照组平均荧光强度值（743．15±34．78）nmol／L相比，差异有统计学意义（P〈0．01，n=7）；同时H2O2（0．3mmol／L）使予给TFA（20mg／kg）组乳鼠心肌细胞的[Ca^2＋]i平均荧光强度值由（668．29±41．15）nmol／L下降到（649．31±39．17）nmol／L，差异无统计学意义（P〉0．05，n=7）。结论H2O2可明显升高乳鼠心肌细胞游离钙离子浓度，黄芪总黄酮可对抗H2O2的这种作用。

Objective To study the effect of TFA on intracellular free calcium concentration of cultured ventricular myocytes. Methods The fluorescent Ca^2＋ indicator Fluo-3 was used to quantitate the calcium signal directly in the primary cultured myocardial cells by LSCM continuous scanning meanwhile recording the intracellular free calcium concentration of cultured ventricular myocytes after administered H2O2. Results The intracellular free calcium concentration was increased significantly Compared with control group, after administered H2O2. The intracellular free calcium average concentration from （743.15±34.78）nmol/L to （1346.89±65.36）nmol/L（P〈0.01,n= 7） ; meanwhile, the intracellular free calcium concentration of cultured ventricular myocytes of the group of TFA（20 mg/kg）show no significant difference after administered（P〉0.05,n=7）. Condusion The above results suggested that H2O2 increase intraeellular free calcium concentration of cultured ventricular myocytes.It＇s effects may antagonized by TFA.