有色氧化铝玻璃复合体的细胞毒性试验和敏感试验

Toxicological test and delayed hypersensitivity reaction of colored alumina-glass composite in guinea-pigs

目的:研究有色氧化铝玻璃复合体修复体的细胞毒性反应和皮肤致敏性。方法:采用分子滤过法对有色氧化铝玻璃复合体进行细胞毒性评价,通过豚鼠皮内注射试验试液,经诱发和加强及过敏激发后观察局部组织的过敏现象。结果:在细胞材料相接触区域试验滤纸的外观呈现蓝染,与其它单层细胞区域相比较,染色密度无差异。细胞毒性评级为0级。20只豚鼠均未见有明显红斑或水肿发生,评定有色氧化铝玻璃复合体的小鼠皮肤过敏率为0%。结论:有色氧化铝玻璃复合体材料无细胞毒性和皮肤致敏性。

Objective： To investigate the toxicological activity and delayed hypersensitivity reaction of alumina-glasscomposite. Methods： The filter diffusion test was adopted to demonstrate the nonspecicic cytotoxicity of test materials after diffusion through a cellulose acetate filter. After the materials were hypodermically injected to the guinea pig, the hypersensitivity reaction was observed after inducement, enhancement and delayed hypersensitivity. Results： There was no difference in staining intensity across the filter between the experimental and control groups. The scale of assessment was 0 （noncytotoxic）. No obvious erythema or edema were observed. The percent of hypersensitivity reaction of alumina-glass composite was 0%. Conclusion： Alumina-glass-composite is a safe and reliable material for prosthetic application.