71例急性白血病免疫表型分析

IMMUNOPHENOTYPIC ANALYSIS OF 71 PATIENTS WITH ACUTE LEUKEMIA

使用间接免疫荧光法对７１例急性白血病（ＡＬ）患者的白血病细胞进行了免疫表型分析。结果显示：２９例ＡＬＬ中，白血病细胞只有淋系相关抗原表达者２３例，同时表达淋髓两系相关抗原者５例，既无淋系亦无髓系抗原表达者１例；４２例ＡＭＬ中，髓系相关抗原阳性者３９例，其中１２例同时表达淋系相关抗原；无髓系抗原表达者３例。结果提示通过对白血病细胞表面分化抗原检测不仅有助于ＡＬ的免疫学分型，而且表明ＡＬ时抗原表达的复杂性。

Immunophenotypic features of 71 patients with acute leukemia(AL) were analysed by a standard indirect immunofluorescence assay using a panel of 18 monoclonal antibodies. The result showed that 23 patients with ALL blasts from 29 patients (29.3%) were positive for lymphoid antigens, but 5 (17.2%) were positive for both lymphoid and myeloid associated antigens, and 1 was negtive for both. In 42 patients with AML(M2 to M6), blasts from 39 cases were positive for myeloid antigens, in which 12 cases were simultaneously expressing lymphoidassociated antigens. In addition, the other 3 cases with AML were negtive for myeloid associated antigens. These findings indicate that patients with AL could be immunophenotypically diagnosed by detecting cell-surface differentiative antigens on blasts, meanwhile demonstrating the complexity of antigen expression on the surface of leukemic cells.