短小芽孢杆菌固态发酵生产木聚糖酶的培养条件优化

Optimization of culture conditions for xylanase production by the solid-state fermentation with Bacillus pumilus

采用单因素试验和正交试验的方法研究了短小芽孢杆菌固态发酵生产木聚糖酶的培养条件,确定了利用麸皮作为在主要基质进行固态发酵生产木聚糖酶的适宜条件:pH 9、温度35℃、料液比1∶2、接种量10%。在500mL三角瓶中固态培养72h左右,木聚糖酶的活力可达4915U/g干曲。

The optimal culture condition for xylanase production by solid-state fermentation with Bacillus pumilus was determined by the single-factor tests and orthogonal experiment. With the wheat bran applied as the base material, the optimal temperature and initial pH for the production of xylanase were found to be 25℃ and pH 9, respectively. The optimal ratio of moisture of wheat bran to mineral salt solution was 1 ： 2 （w/v）. And optimal inoculum was 10%. Using optimized medium, maximum xylanase activity in 72 h by solid-state fermentation in 500 mL flask was 4915 U/g dry koji.