石墨炉原子吸收法测定富硒酸奶中有机硒

Determination of the organic selenium in selenium-enriched yogurt with graphite furnace atomic absorption spectroscopy

采用超声波破碎，离心、透析的方法将有机硒与无机硒分开，利用微波消解法进行样品消化，减少了消化过程中硒的损失。利用石墨炉球子吸收法对富硒酸奶中有机硒的含量进行测定。用1％Ni（NO3）2作为基体改进剂，灰化温度为900℃，原子化温度为2200℃时，测定甯硒酸奶中有机硒含量，该方法检出限为2．5μg/L,精密度为3．24％～8．27％（RSD），加标回收率为98．5％～101.3％。该法具有简便、快速、灵敏、稳定、准确等优点，适于富硒酸奶中有机硒的分析测定。

The organic selenium and inorganic selenium were separated with uhrasonication, centrifugal and dialysis method. The samples were di- gested by using microwave digestion, which could reduce the loss of selenium in digestion. The content of organic selenium in selenium-enriched yogurt was determined by graphite furnace atomic absorption spectroscopy, in which 1% Ni （ NO3 ） 2 was used as matrix modifier, ashing tempera- ture was 900℃, and atomization temperatures was 2200℃. Under these conditions, the limit of detection reached 2.5 μg/L, and the relative stand- ard deviation （RSD） and recovery rate were 3.24% - 8.27% and 96.5%- 101.3% , respectively. The method was suitable for the determination of organic selenium in selenium-enriched yogurt with the advantages of being simple, rapid, sensitive, accurate and stable etc.