阿尼西坦对老龄大鼠脑内单胺类递质及其代谢产物的作用

EFFECT OF ANIRACETAM ON MONOAMINES AND THEIR METABOLITES IN AGED RAT BRAIN

目的：从中枢递质的水平研究促智药阿尼西坦的作用机制。方法：采用反相ＨＰＬＣ电化学法测定了给药老龄大鼠脑内单胺类递质及其代谢产物的含量。结果：阿尼西坦５００ｍｇ／（ｋｇ·ｄ）连续ｉｇ５周后，在老龄给药鼠脑组织中（海马、下丘脑、纹状体）ＮＡ含量分别为６９９±１８３、６９６±１２３、３６４±６８ｎｇ／ｇ；ＤＡ含量分别为３２±７、１２２±１５、２９８６±４１６ｎｇ／ｇ和７７±２７ｎｇ／ｇ（大脑皮层），均显著高于老龄对照鼠（Ｐ＜０．０５）。在上述４个区域５ＨＴ含量与老龄对照鼠无显著性差异。结论：阿尼西坦促智作用机理可能与儿茶酚胺类递质的改变有关。

OBJECTIVE:To investigate the possible mechanism of aniracetam(AM) in central transmitter level. METHODS:The monoamines and their metabolites in treated rat brain were determined by a reversed phase HPLCECD. RESULTS:After 5 weeks consecutive treatment with AM (500 mg/(kg·d),po), the NA level in Hippocampus(Hip), Hypothalamus(Hyp), and striatum(Str) were 699±183; 696±123; and 364±68ng/g rats brain tissue respectively. DA level in treated rats' brain were 77±27 (Cerebralcortex, Cer); 32±7(Hip); 122±15(Hyp); 2 986±416(Str)ng/g brain tissue. These data are significantly higher than those in control animals(P<0.05). The 5HT level in Hip, Hyp, Str and Cer showed no significant difference between active and control groups. CONCLUSION:Results obtained suggested that AM elicits nootropic action in rats may be possibly via monoamine mechanism.