沿江化工园区污水处理厂出水对体外培养的大鼠睾丸细胞生殖毒性研究

Toxicity Evaluation of Sewage Treatment Plant Effluent of Chemical Industrial Park Along the Yangtze River on Rat Testicular Germ Cells in vitro

应用基于原代培养的大鼠睾丸细胞体外毒性测试方法,通过支持细胞、生精细胞及间质细胞这3种细胞的形态、活力、胞外乳酸脱氢酶(LDH)活力、细胞早期凋亡坏死率和睾酮分泌量等指标对沿江化工园区污水处理厂出水的生殖毒性进行了研究.结果表明,新开发的沿江化工园区A和省级沿江化工园区B污水处理厂出水有机提取物浓缩后均能造成不同程度的大鼠睾丸细胞生殖毒性,园区A提取物能明显影响支持细胞(p<0.01)、生精细胞(p<0.05)和间质细胞(p<0.05)的细胞活力,影响支持细胞和生精细胞胞外LDH活力(p<0.01),且其毒性明显高于园区B.暴露导致支持细胞处于凋亡早期,而生精细胞比较敏感,已到了凋亡晚期甚至有小团细胞坏死,虽然间质细胞的形态没有显著变化但园区A有机提取物已明显抑制了间质细胞睾酮的分泌(p<0.01).研究表明,通过基于原代培养的大鼠睾丸细胞体外毒性测试方法能够对污水处理厂出水的生殖毒性做出综合评价,该方法可以作为现行国家污水综合排放化学监测的有效补充.

By using rat testicular germ cells in vitro toxicity testing method based on original cells culture, the reproduction toxicity of sewage treatment plant effluent of Chemical Industrial Park along the Yangtze River was evaluated, through cells changes in morphologic, activity and viability parameters. The results showed that both of the effluents from new developed Chemical Industrial Park A and provincial Chemical Industrial Park B contain reproductive toxic substances. The toxicity of Park A has more significant undergone changes in cells activity of sertoli cells （p 〈 0.01 ）, spermatogenic ceils （ p 〈 0.05） and leyding cells （ p 〈 0.05 ）, lactate dehydrogenase activity （p 〈 0.01 ） and testosterone secretion （p 〈 0.01 ） than that of Park B. Sepermatogenic ceils are more sensitive in indicating reproduction toxicity for testicular, compared with leyding cells and sertoli cells. This study demonstrated that, as an indispensable and complementary tool for water quality assessment, rat testicular germ cells in vitro toxicity testing based on original cells culture can be used to comprehensively evaluate the reproduction toxicity of sewage treatment plant effluent, and provide prompt and useful discharge quality information.