新疆南部地区蚊虫中病毒分离和初步鉴定

Isolation and primary identification of viruses in mosquitoes in the south of Xinjiang

目的首次从新疆南部地区蚊虫中分离病毒并完成初步鉴定。方法2005年7—8月在新疆南部地区采集蚊虫13491只，分130管进行研磨，取上清同时感染C6／36和Vero细胞进行病毒分离，获得的病毒分离物进行基因组核酸电泳检测和负染电子显微镜（简称电镜）观察。结果获得42个对C6／36细胞致病变（cytopathic effects，CPEs）而对Vero细胞不致病变的病毒分离物。对这42个病毒分离物进行基因组核酸电泳检测发现其中27个为相似的12节段双链RNA（double stranded RNA，dsRNA）病毒，1个为10节段dsRNA病毒，5个为DNA病毒；电镜观察显示了这3类病毒的形态特点，并且发现基因组核酸电泳检测中未获得分类信息的9个病毒分离物具有相似的形态特点，是另外一类病毒。结论新疆南部地区蚊虫中可能存在多种病毒，并且以具12节段dsRNA的病毒占优势。

Objective To isolate viruses from mosquitoes in the south of Xinjiang and identify these viruses primarily. Methods A total of 13 491 mosquitoes were collected in the south of Xinjiang from Jul to Aug,2005. These mosquitoes were divided into 130 groups and grinded respectively. The superuates were inoculated in C6/36 and Vero cells. Viruses isolated were detected, the genomic nucleic types by electrophoresis of viral genomes and the morphologies observed under electromicroscope. Results All 42 viruses were isolated ,which caused CPEs on C6/36 but not on Vero cells. 27 viruses showed similar genomic profiles with 12 dsRNA segments. 1 virus displayed genomic profile with 10 dsRNA segments. 5 viruses took on similar genomic profiles with about 4 kbp DNA band. 9 viruses did not get any taxonomy information. Electromicroscopic pictures of these viruses revealed that above four types of viruses had distinguished morphologies indicating different virus species. Conclusion There should be several virus species in the mosquitoes in the south of Xinjiang. dsRNA virus with 12 genomic segments should play analysis a predominant role in the south of Xinjiang.