酶联免疫法定量检测幽门螺杆菌抗体的研究

Study on the Level of Anti-Helicobacter Pylori IgG Antibody with Enzyme Linked Immunosorbent Assay

对73例十二指肠溃疡和胃炎进行血清抗HP抗体定量测定,结果以尿素酶试验和胃粘膜涂片同时阳性作为HP阳性标准,判断血清抗体法检测HP阳性符合率,以同样方法判断血清抗体法阴性符合率.在尿素酶和胃粘膜涂片阳性39例中,抗HP-IgG抗体阳性35例,阳性符合率为89%;尿素酶和胃粘膜涂片阴性34例中,30例阴性,阴性符合率为88%,5例在临界值范围.经卡方检验差别无显著性(P>0.05).抗HP-IgG定量方法的测定比定性方法更准确.定量方法可得出具体数据,可以判断患者感染细菌时间长短以及反映机体对HP免疫力强弱.HP血清学检查可用于观察抗HP疗效及HP普查.本操作每次应设阴阳性对照,用标准品作为定量标准,标本定量应在24h内完成,以免HP-IgG效价降低,若放置低温冰箱,应避免反复冻融.

73 patients of duodenal ulcer and chronic gastritis were evaluated for anti-Helicobacter pylori IgG antibody by enzyme-linked immunosorbent assay (ELISA). It was the positive standard of HP infection that urease test and gastric mucosal biopsy were positive together. The results showed that the sensitivity of anti-HP IgG antibody with ELISA method was 89% (35/39), the specificity was 88% (30/34), chi square test (x2 test) was not significantly difference (P >0.05). The conclusion suggested the method used to test anti-HP IgG antibody with ELISA was better than the qulitative methods.