北极黄河站植物根际土壤放线菌分离培养及其鉴定

ISOLATION AND IDENTIFICATION OF ACTINOBACTERIA FROM RHIZOSPHERIC SOIL IN THE ARCTIC YELLOW RIVER STATION

通过稀释涂布平板法,对北极黄河站(挪威新奥尔松地区)7种常见植物根际土壤样品进行放线菌的分离培养。经扩增性rDNA限制性酶切片段分析(ARDRA)及16S rDNA序列分析确定放线菌53株,分别属于8个属,除了优势菌链霉菌属(Streptomyces)和节杆菌属(Arthrobacter)外,还发现了红球菌(Rhodococcus)、糖丝菌(Saccharothrix)、拉塞氏杆菌(Rathayibacter)、微球菌(Micrococcus)、诺卡氏菌(Nocardia)、克里贝拉(Kribbella)等6种稀有菌属。

Using spread-plate technique, actinobacterial strains were isolated from rhizospheric soil of 7 plants collected in the Arctic Yellow River Station（ Ny-Alesund, the Arctic）. 53 actinobacte- ria were indentified by the analysis of ARDRA and 16S rDNA sequences and were shown to represent strains from 8 genera ： Streptomyces and Arthrobacter which is predominant and rare acti- nobacteria Rhodococcus , Saccharothrix , Rathayibacter , Micrococcus , Nocardia , Kribbella.