铝螯合剂对染铝大鼠学习记忆及脑ATP酶影响

Effect of aluminmum chelating agent on learning and memory ability,ATPase activity and essential element in brain of rats exposed to aluminum

目的探讨铝鳌合剂1,2-二甲基-3-羟基-4-吡啶酮(deferiprone,DFP)对染铝大鼠学习记忆、脑组织中三磷酸腺苷酶(ATP)及铝、锌、铜、铁、钙、镁等元素的影响。方法A lC l3染毒W istar大鼠4周后分别给予不同剂量的DFP 2周,进行迷宫实验、测定脑组织ATP酶活力及铝、锌、铜、铁、钙、镁含量。结果铝染毒组大鼠迷宫实验全天总反应时间(TRT)和错误次数显著高于各给药组(P<0.05,P<0.01);铝染毒组大鼠脑组织中Ca2+Mg2+-ATP酶活力显著低于高剂量组(P<0.05),各给药组ATP酶活力与阴性对照组比较差异均无统计学意义;各给药组脑铝含量显著低于铝染毒组(P<0.01),高剂量组恢复至阴性对照组水平;各给药组大鼠脑组织中锌、铜、铁、钙、镁的含量与阴性对照组比较,差异均无统计学意义。结论在一定剂量范围内,DFP通过排铝,恢复ATP酶的活力改善大鼠的学习记忆功能,同时维持脑组织中锌、铜、铁、钙、镁代谢平衡。

Objective To investigate the effect of 1,2-dimethyl-3-hydroxy-4-pyfidone （defedprone, DFP） on learning and memory ability, ATPase activity and the content of aluminum （ Al ）, zinc （ Zn）, copper （ Cu ）, iron （ Pe）, calcium （Ca） and magnesium （Mg） in brain of rats exposed to aluminum. Methods The Wistar rats were administered with aluminum chloride for four weeks, and then given different doses of DFP for two weeks. The maze test was conducted to the rats and the activity of ATPase and the concentration of Al, Zn, Cu, Fe,Ca and Mg in brain tissue were detected. Results The TRT and error frequency of aluminum-exposed group wre remarkably higher than that of the negative control group（ P 〈0.05 ,P 〈0. 01 ）. The Ca^2＋ Mg^2＋ -ATPase activity of aluminum-exposed group were significantly lower than that of the high dose group（ P 〈 0.05 ）, and the ATPase activities in brain of drug administration groups were not significantly different from that of the negative control group. The aluminum concentration in the brain of drug administration groups were significantly lower than that of aluminum-exposed group （ P 〈 0. 01 ） and that of high dose group returned to the level of negative control group. For the Zn, Cu, Fe, Ca, and Mg contents in brain tissue, there was no statistical significance between all drug administration groups and negative control group. Conclusion Within a certain dose range, the DFP can recover the activity of ATPase though effectively promoting the elimination of Al, thereby improve the learning and memory ability in rats while maintaining Zn, Cu, Fe, Ca and Mg metabolism balance in brain.