孕酮对新生鼠缺氧缺血性脑损伤保护作用

Protective effect of progesterone on hypoxic-ischemic brain damage in newborn rats

目的探讨孕酮对缺氧缺血性脑损伤新生鼠神经细胞凋亡及自由基变化的影响。方法90只7日新生Wistar大鼠随机分成3组:假手术组、缺氧缺血组、药物预防组。观察脑组织缺氧缺血性损伤后6,24,48,72 h,5 d细胞凋亡情况,超氧化物歧化酶(SOD)以及谷胱甘肽过氧化物酶(GSH-Px)活性与丙二醛(MDA)含量的变化。结果缺氧缺血组新生鼠脑细胞凋亡率和MDA含量于缺血缺氧后6 h显著高于假手术组(P<0.01),24 h达高峰,以后逐渐降低,72 h仍明显高于假手术组,至5 d时,2组差异无统计学意义。药物预防组神经元凋亡率和MDA含量于缺血缺氧后6,24,48,72 h明显低于缺氧缺血组(P<0.05);而SOD含量和GSH-PX酶活力于缺血缺氧后5 h显著低于假手术组大鼠(P<0.01),24 h下降至最低点,以后逐渐回升,72 h仍明显低于缺氧缺血组(P<0.05)。结论孕酮通过降低新生鼠缺氧缺血时脑细胞凋亡率和MDA含量,升高SOD含量和GSH-PX酶活力,拮抗细胞凋亡和自由基的产生,发挥对缺氧缺血性脑损伤的保护作用。

Objective To study the effect of progesterone on free radical and apoptosis of hypoxic-ischemic brain damage in newborn rats. Methods Ninety 7-day-old neonatal rats were randomly divided into three groups： sham-operated group,hypoxic-ischemic and pretreatment group. The neuron apoptosis,the activity of GSHPx and the content of SOD and MDA were measured at 6h,24h,48h,72h,5d,respectively. Results The neuronal apoptosis and MDA content in hypoxicischemic group were significantly higher than that in the sham-operated group （ P 〈 0. 01 ）, and those measures in pretreatmerit group were significantly lower than that in hypoxic-ischemic group （ P 〈 0.05 ）. The activity of GSH-Px and the content of SOD in hypoxic-ischemic group were significantly lower than that in the sham-operated group and pretreatment group （ P 〈 0. 05 ）. Conclusion Progesterone can decrease the neuronal apoptosis and MDA content,increase the activity of GSH- Px and the content of SOD of hypoxic-ischemic brain damage in newborn rats.