ATPase F1α在人结直肠癌组织和细胞株中的表达及其意义

Expression of ATPase F1α in human colorectal cancer tissues and cell line and its clinical significance

目的:观察人结直肠癌组织和细胞株中ATPaseF1α的表达,并探讨其意义。方法:应用免疫组化EnVision法测定收集自长海医院2007年8月至12月的44例结直肠癌组织及癌旁组织手术切除标本中ATPaseF1α的表达;半定量RT-PCR方法检测8例结直肠癌及癌旁组织中ATPaseF1αmRNA的表达水平。采用免疫荧光法检测ATPaseF1α在人结肠癌Lo-Vo细胞株胞膜的表达;采用CCK-8活细胞计数法检测抗ATPaseF1α抗体对LoVo细胞增殖的抑制作用。结果:44例结直肠癌组织中ATPaseF1α的表达显著高于癌旁组织(P<0.01);44例结直肠癌组织中ATPaseF1α的中高度表达有35例(79.5%),癌旁配对组织的中高度表达为15例(34.1%)。8例结直肠癌组织中ATPaseF1αmRNA的表达明显高于癌旁组织(P<0.01)。ATPaseF1α在结直肠癌组织中的阳性表达率高于患者术前血浆CEA阳性率(P<0.01),而与患者年龄、性别、肿瘤的临床分期、淋巴结转移、远处转移、肿瘤的部位、分化程度等均无关(P>0.05)。结肠癌LoVo细胞株胞膜上可见颗粒状分布的ATPaseF1α,ATPaseF1α抗体对LoVo细胞的增殖活性有明显的抑制作用(P<0.01)。结论:人结直肠癌组织和细胞株均高表达ATPaseF1α,其作为肿瘤抗原,有可能成为结直肠癌靶向治疗的新靶点。

Objective： To observe the expression of ATPase F1α in colorectal cancer （CRC） tissues and in LoVo cells, and to discuss its clinical significance. Methods： Expression of ATPase F1α protein in 64 CRC specimens and their adjacent normal tissues （August 2007 to December 2007, Changhai Hospital） and ATPase F1α mRNA in 8 colorectal cancer tissues and their adjacent normal tissues were examined by immunohistochemistry EnVision assay and RT-PCR, respectively. Expression of ATPase F1α on the cell surface of LoVo cells was observed by immunofluorescence. The inhib- itory effect of anti-ATPase F1α antibody on the proliferation of LoVo cells was evaluated by CCK-8 assay. Results： Ex- pression of ATPase F1α in the 44 CRCs were significantly higher than those in the adjacent normal tissues as detected by immunohistoehemistry （P 〈0.01 ）. Thirty-five samples （79.5%） in 44 CRCs and 15 samples （34.1%） in their adja- cent normal tissues showed moderate to high expression of ATPase F1α. Expression of ATPase F1α mRNA in 8 CRCs was significantly higher than those in their adjacent normal tissues （P 〈 0.01 ）. Positive rate of ATPase F1α mRNA in CRCs was significantly higher than that of CEA in the same patients before surgery （P 〈 0.01 ）. There was no correlation of AT- Pase F1α expression with the age, gender, tumor stage, lymphoid metastasis, remote metastasis, tumor location and tumor differentiation of colorectal cancer （ P 〉 0.05 ）. Expression of ATPase F1α was observed on the cell surface of LoVo cells, and anti-ATPase F1α antibody significantly inhibited the proliferation of LoVo cells （P 〈 0.01 ）. Conclusion： ATPase F1α is highly expressed in colorectal cancer tissues and cell line. As a tumor association antigen, ATPase F1α may serve as a new target in colorectal cancer immunotherapy.