维持性血液透析患者IL-17水平及与CRP关系的探讨

Exploration of IL-17 amount and its correlation to CRP in the patients with MHD

目的:通过观察维持性血液透析(Maintainance hemodialysis,MHD)患者白细胞介素17(Interleukin17,IL-17)、C反应蛋白(C-reactive protein,CRP)、白细胞介素6(Interleukin6,IL-6)、甘油三酯(Triglyceride,TG)水平、相互之间的相关性及其与透析年限的相关性,探讨IL-17与维持性血液透析患者免疫功能异常及微炎症状态的关系。方法:10例健康体检者作为正常对照组;29例透析超过3个月的不同透析年限的维持性透析(Maintainance hemodialysis,MHD)患者外周血。酶联免疫吸附试验(En-zyme linked immunosorbent assay,ELISA)夹心法检测所有血清样本的CRP、IL-6、IL-17水平及生化检查TG水平。结果:(1)患者CRP、IL-17、TG水平较正常对照组明显升高(P<0.01);IL-6水平较正常人无明显差异。(2)患者CRP、IL-6、IL-17及TG与透析年限均无相关性(P>0.05)。(3)IL-17与CRP、TG呈正相关,IL-6与其他指标均无相关性。结论:(1)MHD患者血浆的IL-17水平远远高于正常人,与公认的反应炎症状态的指标CRP有正相关性,但与透析年限无相关性,提示IL-17可能参与了MHD患者免疫功能异常和微炎症状态的免疫发病机制;(2)IL-17水平和IL-6无相关性,引起MHD患者IL-17升高的机制有待进一步探讨。

Objective：To observe the changes of, and correlation between 1L-17,CRP,IL-6,TG and dialysis duration for exploring the relation between IL-17, immtme anomaly and micro-inflammation in patients with maintainance hemodialysis（MHD）. Methods： Ten sera from normal people and twenty-nine sera from patients hemodialyzed for more than three months were sampled. Serum CRP, IL-6 and IL-17 were measured in all the sera with sandwich ELISA. Results： （ 1 ）Serum CRP, IL-17 and TG in patients were much higher than those in normal group （ P 〈 0.01 ） ;While IL-6 were not statistically different with that in normal group（ P 〉 0.05）. （2） CRP, IL-6, IL-17 and TG amounts in 29 patients undergoing maintenance of dialysis were not correlated to their dialysis duration （months） （ P 〉 0.05）. （3） IL-17 was positively correlat- ed to CRP（ r = 0.460, P 〈 0.01 ）and TG（ r = 0.315, P 〈 0.01） ;Wheras IL-6 was not correlated to IL-17, CRP and TG （ P 〉 0.05 ）. Conclusion： （1） IL-17 in patients is much higher than that in normal people, and is positively correlated to CRP, the acknowledged marker of inflammation status, but not correlated to dialysis duration, suggesting that IL-17 plays a role in pathogenesis of immune anomaly and micro-inflammation in patients with ESRD and HD; （2） There is no correlation between IL-6 and IL-17, and the mechanism of IL-17 elevation in ESRD patients needs to be further investigated.