摘要
目的:建立乳癖安消片中盐酸小檗碱的含量测定方法。方法:采用高效液相色谱法测定,用C18柱(150×4.6mm,5μm),以乙腈:0.1%磷酸(50:50)为流动相,检测波长265nm。结果:盐酸小檗碱的线性范围为0.172μg~1.29μg;相关系数r=0.9995,平均回收率为98.92%,RSD=1.11%(n=5)。结论:所用方法测定样品灵敏度高,重复性好,能有效地控制乳癖安消片的质量。
Objective:To establish method for the determination of berberine in Rupi Anxiao Plan; Methods:The separation was carried out on C 18 ( 150 ×4.6mm, 5 μm) column; The mobile phase was composed of acetonitrile- H3 PO4 (50: 50 ), Detection wavelength was 265 nm; Results: The linear range of berberine was 0.172μg - 1.29μg ( r = 0.9995 ) ; Average recovery was 98.92 % ( RSD = 1.11% ; n = 5 ). Conclusion: This method was accurate,rapid,reproducible and suitable for the quality control of this preparation.
出处
《光明中医》
2009年第3期442-443,共2页
GUANGMING JOURNAL OF CHINESE MEDICINE