气传致敏真菌与支气管哮喘相关性研究

Study on relation of fungi allergenicity to intracutaneous test and bronchial astma

目的研究气传致敏真菌与过敏性哮喘的关系。方法采用空气中暴片调查法和暴皿调查法,了解本地区气传真菌的品种和数量。应用5组多价真菌变应原对346例支气管哮喘患者进行了皮内试验。应用放射变应原吸附试验方法对真菌皮试阳性哮喘患者89例和50例健康者进行血清特异性IgE测定。结果346例支气管哮喘患者真菌变应原皮内试验阳性89例(25.75%)。89例真菌变应原皮内试验阳性患者中特异性IgE阳性78例,真菌变应原皮内试验与sIgE两种检测方法一致率87.64%(78/89),两种检测方法经一致性卡方检验,2χ=9.09,P<0.01。对照组50例中仅2例阳性反应(4.0%),余均为阴性。两组结果经卡方检验,2χ=89.98,P<0.001。结论气传致敏真菌是过敏性哮喘的重要致敏原,采用体内外检测方法联合测定真菌过敏原具有良好的相关性。

Objective To study on relation of fungi allergenicity to intracutaneous test and serum specific IgE in patients with bronchial asthma. Methods Airborne fungi were isolated and identified by using survey mathods of sheet glass and culture plate of exposure determination. Fungi allergen skin test was performed on 346 cases with bronchial asthma. Serum specific IgE was given to patients who showed positive results on the skin test and control group. Results 78 of 89 cases with bronchial asthma （ 87.64% ） showed positive specific IgE assay. Serum specific IgE significantly higher than that from normal control （χ^2 = 89. 98, P 〈 0. 001 ）, with concordance of two tests （ allergen skin test and serum sIgE ） in bronchial asthma （χ^2 = 9.09, P 〈 0. 01 ）. Conclusion The airborne fungi are important sensitization allergens of bronchial asthma with concordance of both the allergens skin test and serum sIgE.