摘要
以油茶的成熟叶片为材料提取DNA,40个样品用改良的提取纯化方法成功提取出适用于AFLP分析的高质量DNA,OD260/OD280在1.7-2.0之间,可以用于AFLP反应。该方法打破了仅能用幼嫩叶片作为提取材料的限制,具有更广泛的适用性和重要的实用性。
DNA of samples was extracted and purified from mature leaves, 40 samples were succeed to extracted high quality DNA by using reformative extraction method. OD260/OD280 value is between 1.7 and 2.0, DNA is suitable for AFLP reaction. This method has broken the limitation of using tender leaves only for DNA extraction, which has wider range applicability and great practical significance.
出处
《广东林业科技》
2009年第1期23-26,共4页
Forestry Science and Technology of Guangdong Province
基金
广东省自然科学基金项目(06025809)
国家"十一五"科技支撑项目(2006BAD18B0204)
关键词
油茶
DNA提取
AFLP反应
Camellia oleifera Abel, DNA extraction, AFLP reaction
