左旋盐酸精氨酸诱导重症急性胰腺炎模型的建议

Advices on establishment of severe acute pancreatitis model induced by L-arg HCl in mice

目的建立一种稳定的小鼠重症急性胰腺炎模型。方法C57BL/6雄性小鼠随机分5组:A组(NS,20只),B组(L-arg HCl2g/kg×2次,30只),C组(L-arg HCl3.5g/kg×2次,30只),D组(L-arg HCl4g/kg×2次,30只),E组(L-arg HCl4.5g/kg×2次,20只)腹腔注射给药,两次间隔时间为1h,A组腹腔注射等体积的无菌生理盐水。观察其死亡率、胰腺湿重/体重比、血清淀粉酶活性、胰腺病理评分和PCNA染色的变化。结果A、B、C三组小鼠无死亡,D组死亡率50%,E组死亡率100%。C、D两组淀粉酶活性第3天升至最高,胰腺湿重/体重比、病理评分、PCNA积分与A组比均有显著性差异(P<0.05);而A、B两组间无显著性差异。结论适当剂量L-盐酸精氨酸腹腔注射能诱导C57BL/6小鼠产生重症急性胰腺炎。该模型制备方法简单,无创伤性,模型稳定,重复性好。

Objective To establish a mouse model of severe acute pancreatitis. Methods The C57BL/6 male mice were randomly divided into 5 groups ： group A （ NS, n = 20）, group B （ L-arg HCl 2 g/kg × 2, n = 30）, group C （ L- arg HC13.5 g/kg×2, n=30）, group D （L-arg HC14 g/kg×2, n=30） and group E （L-arg HC14.5 g/kg×2, n= 20）. All mice received intraperitoneal injection twice at one hour interval. The severity of acute necrotizing pancreatitis was evaluated in terms of mortality, pancreatic wet weight/body weight, serum amylase, histological scores and PCNA changes. Results All mice in group A, B and C survived. The mortality in group D and E were 50% and 100% respectively. The peak level of all parameter changes either in group C or group D were observed at the same time point （72 h after first injection）. Compared with group A, pancreatic wet weight/body weight, histological scores and PCNA changes were significantly different in group C and group D, but they were no significantly different between group A and group B. Conclusion Intraperitoneal injection of proper dosage L-arg HCl can induce severe acute pancreatitis in C57BL/6 mice. This method is simple, noninvasive, and reproducible.