中药方剂回阳救急汤对小鼠免疫力的影响

Influence of huiyangjiouji decoction on murine immune responses

目的探索中药方剂回阳救急汤对小鼠免疫力的影响。方法用卵核蛋白1∶20作为抗原给正常小鼠腹股沟皮下注射作为基础免疫后,中药组当日起每日灌服回阳救急汤药液,对照组当日起每日灌服5%葡萄糖液体,7 d后再用卵核蛋白1∶100作为抗原进行加强免疫,再7 d后用酶联免疫吸附测定(ELISA)法检测相应抗体生成水平。结果中药组小鼠的抗体水平没有显著升高,与对照组比较差异无统计学意义(P>0.05)。结论正常小鼠在强抗原刺激下免疫反应增强,而回阳救急汤药液对小鼠免疫应答没有明显的调节作用。

Objective To investigate the effect of huiyangjiouji decoction on routine immune responses. Methods Mice was subcutaneously immunized with 1：20 diluted egg nucleus protein. The seeond immunization was conducted 7 days after the first immunization but with 1： 100 diluted egg nucleus protein. Huiyangjiouji decoction was orally administered daily for seven days in experimental group; while 5% glucose was given in control group. ELISA was used to examine anti - egg nucleus protein antibody on day 14 postimmunization. Results Compared with control group, antibody level was not significantly increased in experimental group （ P 〉 0.05 ）. Conclusion The immune response was enhanced after the stimulation of the antigen of egg nucleus protein in normal mice. Huiyangjiouji decoction does not show significantly modulatory effect on murine immune system.