灯盏花素改善大鼠局灶性脑缺血后空间学习记忆功能的研究(英文)

Breviscapine Improves Functions of Spatial Learning and Memory of Focal Cerebral Ischemia Rats

目的:研究灯盏花素对大鼠局灶性脑缺血后空间学习记忆能力的影响。方法:线栓法制作大鼠左侧大脑中动脉阻塞模型,腹腔注射灯盏花素(20、40 mg/kg),1次/天,持续2周,缺血对照组注射同体积10%葡萄糖注射液。其后,进行5天的Morris水迷宫实验检测动物的空间学习记忆能力。结果:与假手术组比较,缺血对照组动物在定向航行实验和空间探索实验中均显现了较为严重的空间学习记忆能力障碍。在定向航行实验中,灯盏花素组动物的平均潜伏期较缺血对照组明显缩短(低剂量组,P<0.01;高剂量组,P<0.05),在空间探索实验中,与假手术组(P<0.01)和灯盏花素组动物(P<0.01)比较,缺血对照组动物在非原平台象限停留时间明显延长,穿越原平台位置的次数明显减少。Nissl组织化学染色和ChAT免疫组织化学染色显示,灯盏花素组动物缺血侧皮质神经元数量和ChAT免疫阳性神经元数量较缺血对照组动物明显增多(P<0.01)。在海马CA区的锥体细胞层,神经元数量和ChAT免疫阳性神经元数量在假手术组、缺血对照组和灯盏花素组之间无明显差异。结论:研究结果表明,灯盏花素可以改善大鼠局灶性脑缺血后空间学习记忆能力障碍,保护新皮质内的神经元/胆碱能神经元可能是其保护作用的机制之一。

Objective ： To investigate the effects of breviscapine on the functions of spatial learning and memory of focal cerebral ischemia rats. Methods：Rots withl the left middle cerebral artery occluded were made by an intraluminal filament. Then breviscapine （20 mg/kg,40 mg/kg） in experimental group and 10% glucose in control group were administered intraperitoneally once a day for 2 weeks, and the Morris water maze tasks were carried out for 5 days. Results ： Compared with sham-operation group, the animals of ischemia-control group exhibited seriously impaired spatial learning and memory in both place navigation test and spatial probe test. In the place navigation test,the mean value of escape latency in breviscapine group was significantly shorter than that in ischemia control group （P 〈0. 01 for lower-dose and P 〈 0. 05 for higher-dose breviscapine group, respectively）. In the spatial probe test, compared with sham-operation （P 〈0. 01 ） and breviscapine group （P 〈 0, 01 ）, the rats of ischemia-control group spent more time in the no-former platform quadrant, and showed reduced frequency of crossing former platform site significantly. The numbers of neurons with Nissl staining and choline acetyltransferase （CHAT） immunopositive neurons in ipssilateral cortex in the breviscapine group were significantly more than those in the ischemia-control group （P 〈 0. 01 ）. In hippocampus,the numbers of neurons with Nissl staining and ChAT im- munopositive neurons in the stratum pyramidale of CA area were similar among the groups. Conclusion：These results indicate that breviscapine can improve the functions of spatial learning and memory of focal cerebral ischenfia rats and the protection against the loss of ChAT immunopositive neuron in new cortex may be involved in its mechanisms.