热毒清口服液抑制呼吸道合胞病毒的体外实验研究

Study on the Inhibitory Effect of Reduqing Oral Liquid on Respiratory Syncytial Virus in vitro

目的:评价热毒清口服液体外抑制呼吸道合胞病毒(respiratory syncytial virus,RSV)的效果。方法:采用MTT法及细胞病变抑制实验,以病毒唑为阳性对照,测定热毒清口服液在Hep-2细胞中对呼吸道合胞病毒的抑制作用。结果:热毒清口服液的半数中毒浓度(TC50)为9182.4 mg/L,抑制RSV的半数有效浓度(EC50)为559.8 mg/L,治疗指数(TI)为16.4。结论:热毒清口服液在体外实验中对呼吸道合胞病毒有显著抑制作用。

Objective： To explore the antiviral effect of Reduqing Oral Liquid on respiratory syncytial virus （RSV）. Methods： The anti-RSV effect of Reduqing Oral Liquid in Hep-2 cell culture was investigated by the cytopathic effect （CPE） inhibition assay and MTT assay, with ribavirin as the positive control drug. Results： Reduqing Oral Liquid was effectively antiviral agent for RSV in a concentration-dependent manner in vitro. The median toxic concentration （TC50） was 9182.4 mg/L, the median effective concentration （EC50） was 559. 8 mg/L and the treatment index （TI） was 16.4. Conclusion： Reduqing Oral Liquid has obvious inhibitory effect on RSV in vitro.